Real time optical Biopsy: Time-resolved Fluorescence Spectroscopy instrumentation and validation

Real time optical Biopsy: Time-resolved Fluorescence Spectroscopy instrumentation and validation

The Time-resolved fluorescence spectroscopy (TR-FS) has the potential to differentiate tumor and normal tissue in real time during surgical excision. In this manuscript, we describe the design of a novel TR-FS device, along with preliminary data on detection accuracy for fluorophores in a mixture. The instrument is capable of near real-time fluorescence lifetime acquisition in multiple spectral...

Time-resolved fluorescence spectroscopy

Time-Resolved Fluorescence Spectroscopy with LabView

1.1 Fluorescence phenomenon The absorption and subsequent emission of light by organic and inorganic specimens is typically the result of physical phenomena known as luminescence, which occurs at electronically excited states. Luminescence is formally divided into two categories: fluorescence and phosphorescence, depending on the nature of the excited state. Fluorescence occurs when a photon ex...

Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis.

A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characteri...

Time-resolved optical spectroscopy of wood.

We have proposed and experimentally demonstrated that picosecond time-resolved optical spectroscopy in the visible/near-infrared (NIR) region (700-1040 nm) is a useful technique for noninvasive characterization of wood. This technique has been demonstrated on both softwood and hardwood samples treated in different ways simulating the aging process suffered by waterlogged woods. In all the cases...